GFP Fluorescence Filter Set
The green fluorescent protein ( GFP ) is a protein composed of 238 amino acid residues that exhibits bright green fluorescence when exposed to light in the blue to ultraviolet range. It is can be excited by the 488 nm laser line and is optimally detected at 510 nm.
The biggest advantage of GFP is that it can be heritable, depending on how it was introduced, allowing for continued study of cells and tissues it is expressed in. Visualizing GFP is noninvasive, requiring only illumination with blue light.
To date, GFP has been expressed in many species, including bacteria, yeasts, fungi, fish and mammals, including in human cells.
| Filter Type | Fluoresence Filter Set | ||
| Coating Type | Hard Coated | ||
| Part Number | GFP-BP01A | ||
| Individual Filter | Excitation Filter | Emission Filter | Dichroic Mirror |
| BP-EX03A | BP-EM03A | BP-DM03A | |
| CWL/FWHM | 472/30nm | 520/35nm | 495nm |
| Angle of Incidence | 0±5° | 0±5° | 45° |
| Transmission | >80% | >80% | >90% |
| Size(mounted) | Ø25mm x 5mm | Ø25mm x 3.5mm | Ø25.2mm x 35.6mm x 1.05mm |
| Wavelength Tolerance | ±3nm | ||
| Blocking | OD>5 | ||
| Scratch-Dig | 60-40 | ||
| Substrate | Fused Silica, B270 | ||
| Compatible Microscopes | Most common Nikon, Olympus, and Zeiss Microscopes | ||
| Compatible Fluorophore | 3,3′-diheptyloxacarbocyanine iodide (DIOC7(3)),CopGFP,DIOC7(3)
(3,3′-diheptyloxacarbocyanine iodide),EmGFP (Emerald), GFP (S65A),GFP (S65C),GFP (S65L). |
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