Cy3 Fluorescence Filter Set
Cy3 dye is a bright, orange-fluorescent dye that can be excited using the 532 nm laser line and visualized with TRITC (tetramethylrhodamine) filter sets. In addition to immunocytochemistry applications, Cy3 dye is commonly used to label nucleic acids.
The Cy3 fluorophore is also the basis for the lipophilic neuronal and long-term DiI cell tracing reagents, which append alkyl tails (≥12 carbons) onto the core fluorophore.
| Filter Type | Fluoresence Filter Set | ||
| Coating Type | Hard Coated | ||
| Part Number | Cy3-BP01A | ||
| Individual Filter | Excitation Filter | Emission Filter | Dichroic Mirror |
| BP-EX10A | BP-EM10A | BP-DM10A | |
| CWL/FWHM | 531/40nm | 593/40nm | 562nm |
| Angle of Incidence | 0±5° | 0±5° | 45° |
| Transmission | >80% | >80% | >90% |
| Size(mounted) | Ø25mm x 5mm | Ø25mm x 3.5mm | Ø25.2mm x 35.6mm x 1.05mm |
| Wavelength Tolerance | ±3nm | ||
| Blocking | OD>5 | ||
| Scratch-Dig | 60-40 | ||
| Substrate | Fused Silica, B270 | ||
| Compatible
Microscopes |
Most common Nikon, Olympus, and Zeiss Microscopes | ||
| Compatible
Fluorophore |
Astrazon Red 6B,B Phycoerythrin,BODIPY® R,BODIPY®
TMR-X,Calcium Orange™,CellTracker™ Green Bodipy®,Ethidium Bromide,Evans Blue,FluoSpheres® Nile Red,Genacryl Brilliant Red B,Hexidium Iodide. |
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