DAPI (4′,6-diamidino-2-phenylindole) is a blue-fluorescent DNA stain that binds strongly to A-T rich regions in DNA. As DAPI can pass through an intact cell membrane, it is commonly used as a nuclear counterstain in fluorescence microscopy, flow cytometry, and chromosome staining.
| Filter Type | Fluoresence Filter Set | ||
| Coating Type | Hard Coated | ||
| Part Number | DAPI-BP02A | ||
| Individual Filter | Excitation Filter | Emission Filter | Dichroic Mirror |
| BP-EX14A | BP-EM14A | BP-DM14A | |
| CWL/FWHM | 365/47nm | 445/50nm | 395nm |
| Angle of Incidence | 0±5° | 0±5° | 45° |
| Transmission | >80% | >80% | >90% |
| Size(mounted) | Ø25mm x 5mm | Ø25mm x 3.5mm | Ø25.2mm x 35.6mm x 1.05mm |
| Wavelength Tolerance | ±3nm | ||
| Blocking | OD>5 | ||
| Scratch-Dig | 60-40 | ||
| Substrate | Fused Silica, B270 | ||
| Compatible Microscopes | Most common Nikon, Olympus, and Zeiss Microscopes | ||
| Compatible Fluorophore | DPH, AMCA-X, MEQ, AMC,AMCA,DMACA,
4-Methylumbelliferone,6-Amino-Quinolin, 7-Hydroxy-4-Methylcoumarin,ß-methlyumbelliferone, ABQ,Alexa Fluor® 350,Aminocoumarin, Bis-aminophenyl-oxadizole,Bisbenzamide (Hoechst 33258), Blue GFP,CellTracker™ Blue CMAC,Coumarine maleimide (CPM). |
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