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FITC single band Fluorescence Filter Set
FITC Fluorescence Filter Set
Fluorescein isothiocyanate (FITC) is the original fluorescein molecule functionalized with an isothiocyanate reactive group (-N=C=S), replacing a hydrogen atom on the bottom ring of the structure. It is the most common fluorescent reagents for biological research because of their high absorptivity, excellent fluorescence quantum yield, and good water solubility.FITC has excitation and emission spectrum peak wavelengths of approximately 495 nm/519 nm.
GFP single band Fluorescence Filter Set
GFP single band Fluorescence Filter Set
The green fluorescent protein ( GFP ) is a protein composed of 238 amino acid residues that exhibits bright green fluorescence when exposed to light in the blue to ultraviolet range. It is can be excited by the 488 nm laser line and is optimally detected at 510 nm. The biggest advantage of GFP is that it can be heritable, depending on how it was introduced, allowing for continued study of cells and tissues it is expressed in. Visualizing GFP is noninvasive, requiring only illumination with blue light. To date, GFP has been expressed in many species, including bacteria, yeasts, fungi, fish and mammals, including in human cells.
Texas Red single band Fluorescence Filter Set
Texas Red Fluorescence Filter Set
Texas Red is a red fluorescent dye , used in histology for staining cell specimens, for sorting cells with fluorescent-activated cell sorting machines, in fluorescence microscopy applications, and in immunohistochemistry . Texas Red fluoresces at about 615 nm, and the peak of its absorption spectrum is at 589 nm. Solutions can be excited by a dye laser tuned to 595-605 nm, or less efficiently a krypton laser at 567 nm. It is commonly used in molecular biology techniques like quantitative RT-PCR and cellular assays.
TRITC single band Fluorescence Filter Set
TRITC Fluorescence Filter Set
Tetramethylrhodamine (TRITC) is the base rhodamine molecule functionalized with an isothiocyanate group ( -N=C=S ), replacing a hydrogen atom on the bottom ring of the structure. It is a bright orange-fluorescent dye with excitation ideally suited to the 532 nm laser line. It is commonly conjugated to antibodies and proteins for cellular imaging applications.
